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Log In Sign Up. Add Social Profiles Facebook, Twitter, etc. Uptake of S- 3-Aminooxopropyl -cysteine by Caco-2 Cells more. Acrylamide is a reactive neurotoxin with a high intestinal bioavailability.
Recently we have shown that under the pH regime of the gut acrylamide can react with proteins and that this reaction reduces the uptake of acrylamide in a gut Recently we have shown that under the pH regime of the gut acrylamide can react with proteins and that this reaction reduces the uptake of acrylamide in a gut model.
On the other hand, using radioactive labeled acrylamide, Bjellaas et al. Therefore, we tested whether intestinal proteases can degrade a protein with acrylamide bound to cysteine residues.
Furthermore we tested whether the product of this reaction, S- 3-aminooxopropyl -cysteine, can pass the intestinal barrier. Here we showed that S- 3-aminooxopropyl -cysteine is indeed a product of proteolytic degradation of acrylamide-treated proteins.
Using Caco-2 cells as a gut model, we further showed that the non-protein amino acid S- 3-aminooxopropyl -cysteine is a substrate for the neutral and cationic amino acid transporter system.
Hence we concluded that protein-bound acrylamide can be released in the intestine and that the resulting product S- 3-aminooxopropyl -cysteine is transported through the intestinal barrier and later excreted via the urine.
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We provide an overview and discussion of the methodology currently used to assess the acute and chronic i. The methodology follows guidance published by the European Food Our overview begins with a short introduction including a brief discussion of recent changes to the regulations on which the EFSA Guidance is based.